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Physiological and biochemical analyses after HF model establishment. (A) Ejection fraction (EF) and fractional shortening (FS) were analyzed via ultrasonography (n = 10). (B) The maximum rate of rising/declining LV intraventricular pressure (±dp/dt max), left ventricular end systolic pressure (LVSP) and left ventricular end diastolic pressure (LVEDP) were analyzed via hemodynamic analyses (n = 10). (C) Serum B-type natriuretic peptide (BNP), N-terminal pro-BNP (NT-proBNP) were measured by ELISA. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. the sham group (n = 10). (D) Representative images of cardiac tissue sections following H&E staining. (E) Representative images of cardiac tissue sections following Masson’s trichrome staining.

Journal: Journal of Advanced Research

Article Title: Whole-body mass spectrometry imaging reveals the systemic metabolic disorder and catecholamines biosynthesis alteration on heart-gut axis in heart failure rat

doi: 10.1016/j.jare.2024.09.001

Figure Lengend Snippet: Physiological and biochemical analyses after HF model establishment. (A) Ejection fraction (EF) and fractional shortening (FS) were analyzed via ultrasonography (n = 10). (B) The maximum rate of rising/declining LV intraventricular pressure (±dp/dt max), left ventricular end systolic pressure (LVSP) and left ventricular end diastolic pressure (LVEDP) were analyzed via hemodynamic analyses (n = 10). (C) Serum B-type natriuretic peptide (BNP), N-terminal pro-BNP (NT-proBNP) were measured by ELISA. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. the sham group (n = 10). (D) Representative images of cardiac tissue sections following H&E staining. (E) Representative images of cardiac tissue sections following Masson’s trichrome staining.

Article Snippet: Following weighing and anesthetization, a Millar catheter (SPR-320NR, Millar,USA) was introduced into the left ventricle, and hemodynamic indices were simultaneously recorded using a physiological signal acquisition system (MP160, BIOPAC System, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Staining

Physiological and biochemical analyses of validation experiments. (A) Ejection fraction (EF), fractional shortening (FS), left ventricular internal dimension systole (LVIDs) and left ventricular end-systolic volume (LVESV) were analyzed via ultrasonography in Sham (n = 10), Sham-NE (n = 6), Model (n = 10), Model-NE (n = 9) and MET groups (n = 11). (B) The maximum rate of rising/declining LV intraventricular pressure (±dp/dt max), LV systolic pressure (LVSP) and left ventricular end diastolic pressure (LVEDP) were analyzed via hemodynamic analyses in Sham (n = 10), Sham-NE (n = 6), Model (n = 10), Model-NE (n = 9) and MET groups (n = 11). (C) Serum B-type natriuretic peptide (BNP), N-terminal pro-BNP (NT-proBNP), lipopolysaccharide (LPS) and diamine oxidase (DAO) were measured by ELISA in Sham (n = 10), Sham-NE (n = 6), Model (n = 10), Model-NE (n = 9) and MET groups (n = 11). (D) Representative images of cardiac tissue sections following H&E or Masson’s trichrome staining. (E) Representative images of colonic tissue sections following H&E staining. (F) Relative abundance of s_Turicibacter_sanguinis in Sham, Sham-NE, Model, Model-NE and MET groups. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. the sham group, # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. the model group.

Journal: Journal of Advanced Research

Article Title: Whole-body mass spectrometry imaging reveals the systemic metabolic disorder and catecholamines biosynthesis alteration on heart-gut axis in heart failure rat

doi: 10.1016/j.jare.2024.09.001

Figure Lengend Snippet: Physiological and biochemical analyses of validation experiments. (A) Ejection fraction (EF), fractional shortening (FS), left ventricular internal dimension systole (LVIDs) and left ventricular end-systolic volume (LVESV) were analyzed via ultrasonography in Sham (n = 10), Sham-NE (n = 6), Model (n = 10), Model-NE (n = 9) and MET groups (n = 11). (B) The maximum rate of rising/declining LV intraventricular pressure (±dp/dt max), LV systolic pressure (LVSP) and left ventricular end diastolic pressure (LVEDP) were analyzed via hemodynamic analyses in Sham (n = 10), Sham-NE (n = 6), Model (n = 10), Model-NE (n = 9) and MET groups (n = 11). (C) Serum B-type natriuretic peptide (BNP), N-terminal pro-BNP (NT-proBNP), lipopolysaccharide (LPS) and diamine oxidase (DAO) were measured by ELISA in Sham (n = 10), Sham-NE (n = 6), Model (n = 10), Model-NE (n = 9) and MET groups (n = 11). (D) Representative images of cardiac tissue sections following H&E or Masson’s trichrome staining. (E) Representative images of colonic tissue sections following H&E staining. (F) Relative abundance of s_Turicibacter_sanguinis in Sham, Sham-NE, Model, Model-NE and MET groups. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. the sham group, # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. the model group.

Article Snippet: Following weighing and anesthetization, a Millar catheter (SPR-320NR, Millar,USA) was introduced into the left ventricle, and hemodynamic indices were simultaneously recorded using a physiological signal acquisition system (MP160, BIOPAC System, USA).

Techniques: Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Staining